Ngari has worked for over ten years in molecular biological, clinical and drug discovery research laboratories at The University of Queensland and previously at the Thrombosis Research Institute, London on both grant and industry funded projects. She initially worked for 5 years in pharmaceutical quality control and environmental inorganic industrial laboratories.
Her skills combine molecular and cell biology, biochemistry, primary cell culture, human pluripotent stem cell culture, histology, immunofluorescence and live cell imaging, fluorescent and confocal microscopy, quantitative RT-PCR, transfection of cell lines, cell-based assays, western blotting, molecular cloning, genotyping, amplification and purification of adenoviral stocks, small animal handling and surgery, analytical instrumentation (HPLC and mass spectrometry), peptide chemistry, proteomics and general laboratory management.
Some of the most recent projects that she has worked on before joining the Macrophage Biology laboratory include:
- GPCR Pharmacological characterisation of peptides using Calcium flux FLIPR assay (384 well plate) for Oxytocin and Vasopressin receptors for neuropeptide drug discovery research
- Cardiac myocytes cultivation and purification from neonatal rats
- Adenoviral stock inoculation, amplification and purification to generate large scale preparations of high-titre virus for research on cardiac receptor research
- Cardiac cell differentiation and harvesting from single cell adapted human pluripotent stem cell lines for use in cardiac regeneration tissue engineering projects
- Immunofluorescent staining and confocal analysis of differentiated nasal epithelial cells to define; basal, ciliated, goblet cells and different cell junctions for asthma research.
- Live cell imaging for wound healing investigations on differentiated nasal epithelial cells grown on well inserts using Air Liquid Interface (ALI) culturing techniques.
She has also travelled extensively throughout Africa and worked for 2 years as a technical advisor in a resource poor rural African (Nkhoma, Malawi) hospital laboratory which was a capacity building assignment sponsored by Australian Volunteers International to provide leadership, management and technical training to 8 Malawian laboratory staff to WHO-AFRICA accreditation level while increasing the scope of laboratory services. She lived and worked in the same local community as the team that she mentored.
In: Dis Model Mech, 2022, ISSN: 1754-8411.
In: PLOS Genetics, vol. 17, no. 6, pp. e1009605, 2021, ISSN: 1553-7404.
In: PLOS Genetics, vol. 17, no. 6, pp. e1009605, 2021, ISSN: 1553-7404, (Publisher: Public Library of Science).
In: Neurobiology of Disease, vol. 151, 2021, ISSN: 09699961, (Publisher: Academic Press Inc.).